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Tris
|Section2= |Section6= |Section7= }} Tris or Tris(hydroxymethyl)aminomethane or THAM is an organic compound with the formula (HOCH2)3CNH2. Tris is extensively used in biochemistry and molecular biology.〔Gomori, G., (Preparation of Buffers for Use in Enzyme Studies ). Methods Enzymology., 1, 138-146 (1955).〕 In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes. ==Buffering features== Tris has a p''K''a of 8.07 at 25 °C, which implies that the buffer has an effective pH range between 7.5 and 9.0.
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Tris」の詳細全文を読む
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